Bryan Johnson
Entrepreneur, founder of Blueprint
Bryan Johnson is an entrepreneur who founded the payments company Braintree, sold in 2013, and later the neurotechnology company Kernel. He now runs Blueprint, which sells food, supplements and tests, and publishes his own anti-aging protocol online. He is known for measuring his body in detail and for trying experimental therapies on himself as part of his Don't Die project.
Updated November 1, 2026 · 35 experiments · cited in 7 guides
Their protocol
Blueprint (Bryan Johnson)What it asks others to do, rated by evidence, with the rules card and the studies. Personal experiments are listed below, not in the protocol.What Bryan Johnson has tried
35 experiments we can source: 23 kept, 6 testing, 6 stopped. Trying something is not recommending it; each entry says which.
Kept
AcarboseDrugs
Dates not stated · Does not recommend it to others
Takes it daily to blunt blood sugar spikes after meals and improve insulin sensitivity; his page lists the dose as 200 mg in one place and 400 mg in another.
“helps blunt post-meal blood sugar spikes, improve insulin sensitivity”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Alpha-ketoglutarateSupplements
Dates not stated · Recommends it to others
Drinks a scoop of his Blueprint Longevity Mix every morning, which lists 2,000 mg of calcium AKG per scoop, and his protocol names calcium AKG among the supplements in the Blueprint stack he sells.
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Blood biomarker trackingProcedures
Dates not stated · Recommends it to others
Lists a blood draw every 3 to 6 months under routine measurement and tells readers to measure their biomarkers to judge their diet; his company sells blood biomarker testing.
“Whatever your food preferences, it’s best if you measure your biomarkers to determine if the foods you eat are serving your body.”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
CandesartanDrugs
Dates not stated · Does not recommend it to others
Takes 8 mg a day, citing evidence that it lowers blood pressure, protects against heart failure progression and preserves kidney function.
“evidence that it reduces blood pressure, protects against heart failure progression, preserves kidney function, and possibly reduces brain inflammation”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
CreatineSupplements
Dates not stated · Recommends it to others
Takes 5 g of creatine plus 2.5 g in his Longevity Mix before morning exercise, 7.5 g a day in total, and sells creatine as part of the Blueprint stack.
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
DutasterideDrugs
Dates not stated · Does not recommend it to others
Used a custom topical scalp formula with 0.25% dutasteride, avoiding oral finasteride; when twice-daily use pushed his blood DHT too low, he cut back to once a day.
“I’m back at 1x daily and will retest my blood in 90 days.”
Bryan Johnson, How I Reversed My Hair Loss (October 2024) · Status checked October 18, 2024 · Suggest a correction
Epigenetic age testsProcedures
Dates not stated · Does not recommend it to others
Tracks his pace of aging with the DunedinPACE epigenetic clock and in January 2025 reported 0.57, which he reads as aging about 7 months per calendar year.
“It’s to lower my speed of aging. So I’m currently at 0.57”
Interview Magazine, Bryan Johnson Wants You to Have Better Boners (January 2025) · Status checked January 28, 2025 · Suggest a correction
Fractional laser skin resurfacingProcedures
Dates not stated · Does not recommend it to others
Gets 1927 nm and 1550 nm laser treatments about every 6 months as part of his advanced skin therapies, alongside the Sofwave and Everesse devices.
“1927 nm laser (1x every 6 months, can do more or less depending on your status and goals)”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Hyperbaric oxygen therapyTherapies
Started Nov 2024 · Does not recommend it to others
After 60 sessions at 2 atmospheres he reported lower inflammation and longer telomeres among other changes, calls it one of his highest-value therapies, and keeps it in his routine.
“Hyperbaric oxygen therapy ranks as one of the highest value health therapies I’ve done.”
Bryan Johnson, Bryan's Protocol (checked October 2026) · Status checked October 31, 2026 · Suggest a correction
Intermittent hypoxia-hyperoxia (IHHT)Therapies
Dates not stated · Does not recommend it to others
Does a 42-minute IHHT session in his morning routine on days when he does not do hyperbaric oxygen therapy.
“Either HBOT (60 or 90 min) or IHHT (42 min)”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Large daily supplement stacksSupplements
Dates not stated · Recommends it to others
Takes a daily stack of capsules, powders and oils listed in his protocol, and points readers to the Blueprint stack, which his company sells.
“I’ll take the following pills”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
MinoxidilDrugs
Dates not stated · Does not recommend it to others
Takes 3.75 mg of oral minoxidil a day, after starting at 2.5 mg to check for side effects, and applies 1 mL of 5% topical minoxidil each night.
“I started with 2.5 mg and evaluated for side effects. I now take 3.75 mg a day.”
Bryan Johnson, Bryan Johnson's Haircare Routine (July 2026) · Status checked November 1, 2026 · Suggest a correction
Morning sunlightTherapies
Dates not stated · Recommends it to others
Tells readers to get outside within 15 to 30 minutes of waking; because he is up before the sun, he gets 3 to 4 minutes from a 10,000 lux lamp instead.
“My eyes get light from a 10,000 LUX for 3-4 min as I’m up before the sun”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Multi-omics self-trackingDevices
Dates not stated · Recommends it to others
Calls himself the most biologically measured person ever, and tells readers to measure their own biomarkers, listing blood draws every 3 to 6 months, yearly whole-body MRI after 40 and glucose monitoring, with tests his company sells.
“I am certainly the most biologically measured person ever.”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
PCSK9 inhibitorsDrugs
Dates not stated · Does not recommend it to others
Injects Repatha (evolocumab) 140 mg every two weeks, which his page describes as cholesterol optimization.
“Repatha 140mg/mL every two weeks for cholesterol optimization.”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Red light therapyDevices
Dates not stated · Does not recommend it to others
Does two 6-minute full-body red and near-infrared sessions a day for skin, healing and mitochondrial health, and tells readers without access to start with the basics instead.
“2x 6 min daily (morning and evening routines)”
Bryan Johnson, Bryan's Protocol (checked October 2026) · Status checked October 31, 2026 · Suggest a correction
SaunaTherapies
Dates not stated · Recommends it to others
Uses a dry sauna at about 175°F daily for 20 minutes with an ice pack to protect fertility, and suggests readers aim for 3 to 5 sessions a week.
“Dry sauna: 175F for 20 minutes. I place an ice pack on the boys.”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
SGLT2 inhibitorsDrugs
Dates not stated · Does not recommend it to others
Takes Jardiance (empagliflozin) 10 mg a day, citing evidence that SGLT2 inhibitors work as a longevity intervention.
“good evidence of SGLT-2 inhibitors as a longevity intervention and calorie restriction mimetic from animal experimentation”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Shockwave therapyTherapies
Dates not stated · Does not recommend it to others
Uses 4,500 shocks three days a week on his joints and spine for what he calls proactive rejuvenation; no results reported.
“I’m using it for proactive rejuvenation.”
Bryan Johnson, Bryan's Protocol (checked October 2026) · Status checked October 31, 2026 · Suggest a correction
TadalafilDrugs
Started Jun 2023 · Does not recommend it to others
Takes 5 mg a day for blood vessel function and inflammation, after starting at 2.5 mg in June 2023 as a longevity and bladder health intervention.
“enhances endothelial function, reduces inflammation, and potentially protects against age-related cardiovascular and metabolic decline”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Time-restricted eatingDiets and fasting
Dates not stated · Does not recommend it to others
Starts eating at about 5:30 a.m. and has his final meal at noon, and tells followers to finish eating at least four hours before bed.
“my final meal of the day which will be some combination of veggies, nuts, seeds and berries”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
TretinoinDrugs
Dates not stated · Does not recommend it to others
Uses tretinoin at 0.025 to 0.1% as part of his daily skin routine.
“Tretinoin 0.025-0.1%”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Whole-body MRI screeningProcedures
Dates not stated · Recommends it to others
Lists a full-body MRI every year in his protocol for people over 40 or with a high-risk family history, and has said a routine MRI found narrowed jugular veins that he then treated.
“Full body MRI annually (if over 40 or a family history of high risk)”
Bryan Johnson, Bryan's Protocol (checked November 2026) · Status checked November 1, 2026 · Suggest a correction
Testing
Follistatin gene therapyTherapies
Started Oct 2023 · Does not recommend it to others
A one-time, unapproved follistatin gene therapy from Minicircle in Roatan, Honduras, aimed at more muscle and slower aging; he reported a 160% rise in follistatin and has not reported an end result.
“I am now a genetically enhanced human”
Fortune, Bryan Johnson is a human guinea pig for unproven gene therapy (December 2023) · Status checked October 31, 2026 · Suggest a correction
Mesenchymal stem cell injectionsProcedures
Started Mar 2024 · Does not recommend it to others
A one-time injection of 300 million young donor bone-derived stem cells into his knees, hips and shoulders, with a follow-up MRI planned at 12 months; no results published.
“In March 2024, I had 300 million MSCs injected into my knees, hips and shoulders. Follow up MRI is in 12 months.”
Bryan Johnson, Bryan's Protocol (checked October 2026) · Status checked October 31, 2026 · Suggest a correction
MetforminDrugs
Dates not stated · Does not recommend it to others
Takes 500 mg in 6-week on and off cycles, which he describes as an experiment.
“Metformin 500 mg (Rx) (am experimenting 6-week cycles on/off)”
Bryan Johnson, Bryan's Protocol (checked October 2026) · Status checked October 31, 2026 · Suggest a correction
Penile BotoxProcedures
Dates not stated · Does not recommend it to others
Reported adding Botox injections to the penis, with shockwave therapy, after starting tadalafil, and said they lowered his nighttime erection biological age to around 20.
“nighttime erection biological age to around 20”
People, Why Bryan Johnson gets Botox in his penis (March 2025, via AOL) · Status checked March 28, 2025 · Suggest a correction
PsilocybinDrugs
Started Nov 2025 · Does not recommend it to others
Took about 5 g of psilocybin mushrooms on a livestream, measuring his body before and after to test whether it affects aging; it was the second of three planned trips.
The San Francisco Standard, Bryan Johnson's mushroom livestream (December 2025) · Status checked October 31, 2026 · Suggest a correction
Total plasma exchangeTherapies
Started Oct 2024 · Does not recommend it to others
Has his plasma replaced with albumin and IVIG on a biweekly schedule to test it as a rejuvenation therapy, and says it shows measurable effects.
“my team and I aim to figure out what works best when it comes to the use of TPE as a rejuvenation and longevity therapy”
Decrypt, Bryan Johnson ends blood infusions from son, opts for plasma instead (January 2025) · Status checked October 31, 2026 · Suggest a correction
Stopped
CerebrolysinDrugs
Dates not stated · Does not recommend it to others
Took daily 5 mL injections for three months and measured no effect, noting that no usable biomarker exists to judge it either way.
“Trialed daily 5 mL cerebrolysin injections intramuscularly for cognitive benefits for three months. No effect measured.”
Bryan Johnson, Bryan's Protocol (checked October 2026) · Status checked October 31, 2026 · Suggest a correction
Donor fat injections to the faceProcedures
Started Nov 2024, stopped Nov 2024 · Does not recommend it to others
A donor fat matrix injected to restore facial volume lost on his diet caused a severe allergic reaction that swelled his face until he could not see; it settled after seven days.
“Immediately following the injections, my face began to blow up. And then it got worse, and worse, and worse until I couldn’t even see”
Fortune, Bryan Johnson's anti-aging procedure left him unrecognizable (November 2024) · Status checked October 31, 2026 · Suggest a correction
GLP-1 drugsDrugs
Started Jan 2025, stopped Jan 2025 · Does not recommend it to others
Halted low-dose tirzepatide after about three weeks because his resting heart rate rose 3 bpm, his heart rate variability fell and his sleep quality dropped about 10%.
“it increased my resting heart rate by 3 bpm, lowered my HRV by 7 and decreased my sleep quality by 10%”
Bryan Johnson on X, halting tirzepatide microdosing (January 2025) · Status checked January 30, 2025 · Suggest a correction
PRP for hair lossProcedures
Dates not stated · Does not recommend it to others
Had plasma injections into his scalp for 12 months and saw nearly no benefit, calling the treatment painful, expensive and time-consuming; it is absent from his 2026 routine.
“After diligently doing it for 12 months, we saw nearly no benefit.”
Bryan Johnson, How I Reversed My Hair Loss (October 2024) · Status checked October 18, 2024 · Suggest a correction
RapamycinDrugs
Started 2019, stopped Sep 2024 · Does not recommend it to others
Ended it after skin infections, higher glucose, abnormal lipids and a faster resting heart rate, judging the benefits not worth the side effects.
“With no other underlying causes identified, we suspected Rapamycin, and since dosage adjustments had no effect, we decided to discontinue it entirely”
Scientific American, Silicon Valley's longevity biohackers are engaged in a dangerous experiment (June 2026) · Status checked October 31, 2026 · Suggest a correction
Young plasma exchangeTherapies
Started 2023, stopped Jul 2023 · Does not recommend it to others
Six monthly 1-liter exchanges, one with plasma from his 17-year-old son while his own went to his father, showed no benefit in his biomarkers, so he ended them.
“Does not in my case stack benefit on top of my existing interventions.”
Fortune, Bryan Johnson admits he saw no benefits after injecting his son's plasma (July 2023) · Status checked October 31, 2026 · Suggest a correction
Where our guides cite Bryan Johnson
Their public positions, summarized in our words. Follow each link for the full context.
Blueprint (Bryan Johnson)Follow it
Created and follows the regimen, publishes his routine and biomarker results, and says anyone can get much of the benefit from free habits, while noting that his biological age claims are preliminary.
Creatine supplementationFollow it
His published Blueprint routine includes 5 g of creatine plus 2.5 g in his Longevity Mix, 7.5 g a day in total, taken before morning exercise.
Morning sunlightFollow it
His published protocol tells readers to get outside within 15 to 30 minutes of waking, and he uses a 10,000 lux lamp for 3 to 4 minutes because he wakes before sunrise.
Red light therapyFollow it
Uses red and near-infrared light twice a day in 6 minute sessions and a 655 nm cap for hair, for collagen, healing and mitochondrial health, while telling followers the basics matter more if they lack access.
SaunaFollow it
Uses a dry sauna at 175 °F (about 79 °C)about 79 °C (175 °F) for 20 minutes daily with an ice pack on the groin to protect fertility, and reports personal drops in blood pressure and urinary toxins that have not been tested in trials.
Sleep consistencyFollow it
His protocol says being consistent is as important as getting enough sleep; he goes to bed at 8:30 p.m., wakes around 4:30 a.m., and tells readers to pick any bedtime and stick to it.
Time-restricted eatingFollow it
Follows a very early window, eating from about 5:35 a.m. to a final meal around noon, and his protocol tells followers to eat their last meal at least four hours before bed.